ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Study of 'Harden-Young's' effect. V. Purification of the barium salt of diphosphate ester of D-fructose

A. Vojtková-Lepšíková and A. Kocková-Kratochvílová

Slovak Academy of Sciences, Bratislava

Abstract: cf. CA 52, 7926a.-For purification of the Ba salt of the diphosphate ester of D-fructose, 3 methods were applied: those of Meskova and Severin (Praktikum Po Biochemii Zivotnych, Moscow, 1950), of Robison and MacFarlane (Methoden der Fermentforschung, Leipzig, 1941), and of the authors. Only the authors' method was found satisfactory: The crude Ba salt of phosphorylated D-fructose was dissolved in 5 times amt. of H₂O at pH 4.0 made up with N HCl, mixed well, and cooled. The insol. part was sepd. by filtration and a double amt. of 96% EtOH added to the filtrate. The formed ppt. was filtered off and dried in vacuo over P₂O₅. This procedure was repeated 3 times.

Full paper in Portable Document Format: 159a647.pdf (in Slovak)

Chemical Papers 15 (9) 647–650 (1961)