ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Fabrication of amperometric cholesterol biosensor based on SnO2 nanoparticles and Nafion-modified carbon paste electrode

Gözde Aydoğdu Tığ, Derya Koyuncu Zeybek, and Şule Pekyardımcı

^aDepartment of Chemistry, Faculty of Science, Ankara University, Ankara 06100, Turkey

E-mail: gaydogdu@science.ankara.edu.tr

Abstract: This study reports the fabrication of an amperometric cholesterol biosensor based on cholesterol oxidase (ChOx), SnO₂NPs and Nafion-modified carbon paste enzyme electrodes (CPE/SnO₂NPs-ChOx/Naf). The electrochemical characterisations of BCPE and CPE/SnO₂NPs were performed using CV and EIS. The determination of cholesterol was carried out by electrochemical oxidation of H₂O₂ at 0.6 V vs. Ag/AgCl. The CPE/SnO₂NPs-ChOx/Naf presented a linear range from 0.20 μmol L⁻¹ to 4.95 μmol L⁻¹ with a low limit of detection (0.04 μmol L⁻¹ ). In addition, the optimal values for pH and temperature were found to be 7.5 and 35°C, respectively. The CPE/SnO₂NPs-ChOx/Naf was used for the determination of cholesterol in serum samples and good results were obtained

Keywords: cholesterol biosensor – carbon paste electrode – amperometry – SnO2 nanoparticles

Full paper is available at www.springerlink.com.

DOI: 10.1515/chempap-2016-0005

Chemical Papers 70 (6) 695–705 (2016)