ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Determination of citrate released from stabilized gold nanoparticles by capillary zone electrophoresis

Jana Horská, Juraj Ševčík, and Jan Petr

Palacký University in Olomouc, Olomouc, Czech Republic

E-mail: secjpetr@gmail.com

Abstract: In this article, we report a pilot study on release of citrate ions from citrate-stabilized gold nanoparticles by capillary zone electrophoresis. First, a method for determination of citrate released from nanoparticles was developed using 5 mM phthalate buffer pH 6.5 with polybrene capillary inner wall coating. Within these conditions, citrate migrated in 1.8 min and it can be determined with LOD of 7 µmol/L using molybdate as the internal standard. The release of citrate was initiated by addition of common MOPS buffer ions used in capillary electrophoresis to nanoparticles samples. Non-linear behavior was found that proves release of citrate from stabilized gold nanoparticles. The release is initiated when 5 mM MOPS is added to nanoparticles’ solution. This behavior can partially explain zeta potential change of the nanoparticles from –34 mV in bulk solution to –28 mV in 50 mM MOPS.

Keywords: Capillary zone electrophoresis ; Citrate release ; Gold nanoparticles ; MOPS ; Stabilization 

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-017-0291-8

Chemical Papers 72 (2) 419–424 (2018)