ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Kinetic study of growth, lipid and carotenoid formation in β-carotene producing Rhodotorula glutinis

Jana Tkáčová, Tatiana Klempová, and Milan Čertík

Slovak University of Biotechnology, Bratislava, Slovak Republic

E-mail: milan.certik@stuba.sk

Abstract: The oleaginous and red yeast Rhodotorula glutinis CCY 20-2-26 is known for its high-storage lipids accumulation in cells as well as for β-carotene biosynthesis. The work is focused on the study of lipogenesis and carotenoid pigments formation by the yeast grown in media with four different C/N ratios (20:1, 50:1, 70:1 and 100:1). Total fatty acids accumulation in cells reached the maximal value of 48% and yielded up to 8.9 g fatty acid/L media under C/N 70:1. On contrary, while the highest pigment accumulation in the yeast (1268 μg/g of cells) was found at the C/N ratio of 20:1, the maximal carotenoid yield (mainly β-carotene) of 12.7 mg/L was obtained under 50:1 ratio. Calculation of kinetic parameters of metabolites production revealed the metabolic correlations among glucose consumption, lipogenesis and carotenoid biosynthesis. It was demonstrated that glucose exhaustion resulted in reduction of growth and lipid accumulation in cells. Then, storage fatty acids, especially palmitic and oleic acids, begun to be degraded by β-oxidations and formed acetyl-CoA which was especially used for biosynthesis of β-carotene. This is the first study providing the new hypothesis about the metabolic connection between fatty acids and β-carotene metabolism in the red yeasts.

Keywords: Kinetic analysis ; Rhodotorula ; Β-carotene production ; Impact of C/N ratio ; Optimisation of cultivation conditions 

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-017-0368-4

Chemical Papers 72 (5) 1193–1203 (2018)